rabbit anti microtubule associated proteins 1a 1b light chain 3b  (Proteintech)

Journal: bioRxiv

Article Title: Chronic administration of the hydrogen sulfide prodrug SG1002 partially protects against erectile dysfunction resulting from long-term androgen deprivation

doi: 10.1101/2025.05.26.656242

Figure Lengend Snippet: The expression of proteins related to autophagy in mouse corpus cavernosum measured by western blot. Mice underwent sham surgery (Sham) or were surgically castrated (Cast). Two groups of castrated mice were treated with low-dose (CLS) or high-dose (CHS) SG1002. Proteins include light chain 3B (LC3-II), P-p62, p62, and autophagy-related protein (ATG7). Protein expression was normalized to GAPDH or representative controls. Values represent means ± SEM for n = 12 animals per group. * p < 0.05 compared to Sham.

Article Snippet: Primary antibodies were obtained from Cell Signaling Technologies (CST; Danvers, MA, USA), or Protein Tech (PT; Rosemount, IL, USA) and used at the following dilutions: glutamate-cysteine ligase (Gclc, PT #12601-1-AP, 1:1000), optic atrophy type 1 (Opa1, CST #80471, 1:1000), peroxiredoxin 3 (Prdx3, PT #10664-1-AP, 1:1000), peroxiredoxin 5 (Prdx5, PT #17724-1-AP, 1:1000), mitofusin 1 (Mfn1, PT #13798-1-AP, 1:1000), mitofusin 2 (Mfn2, PT #12186-1-AP, 1:1000), NAD(P)H dehydrogenase quinone 1 (Nqo1, CST #62262, 1:1000), thioredoxin 1 (Trx1, CST #2298, 1:1000), thioredoxin-interacting protein (Txnip, PT #18243-1-AP, 1:1000), thioredoxin 2 (Trx2, CST #14907, 1:1000), dynamin-related protein 1 (Drp1, CST #8570, 1:1000), superoxide dismutase 1 (Sod1, PT #10269-1-AP, 1:1000), superoxide dismutase 2 (Sod2, CST #13141, 1:2000), superoxide dismutase 3 (Sod3, Santa Cruz Biotechnology #sc-271170, 1:1000) autophagy-related protein (Atg7, CST #8558, 1:1000), mitochondrial fission 1 (Fis1, PT #10956-1-AP, 1:1000), phosphorylated (Ser 403 ) Sequestosome-1 (P-Sqstm1/p62, CST #39786, 1:1000), sequestosome-1 (Sqstm1/p62, CST #39749, 1:1000), heme oxygenase 1 (HO-1, PT #10701-1-AP, 1:1000), light chain 3B (LC3B, CST #2775, 1:1000), GAPDH (PT #60004-1-Ig, 1:4000).

Techniques: Expressing, Western Blot